DNA

Part:BBa_K4630112:Design

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)


stgRNA-cassette 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is built for the extension of the recording system. Using Biobrick Assembly, the fragment can be inserted downstream the stgRNA-cassette 1/2. Homologous arm is built for intra-plasmid recombination with Lac promoter in the previous cassette.


Source

Complete synthesis.

References